Vomitoxin hazard

Vomitoxin gets its name because it can cause vomiting reaction in animals. In severe cases, it can cause death of young animals. The acute toxicity of Vomitoxin is related to the species, age, sex and exposure route of animals. Male animals are more sensitive to the toxin. Acute poisoning animals mainly manifested as unstable standing, slow reaction, bristles, loss of appetite, vomiting, etc., and could also cause anti feeding reaction of animals, among which pigs were most sensitive to vomiting toxin.

     After people eat cereals and oils containing vomitoxin, they will suffer from stomach discomfort, dizziness, abdominal distension, headache, nausea, vomiting, hand and foot numbness, general fatigue, facial flushing, food poisoning leukocytopenia and other symptoms. In patients with more serious symptoms, the respiration, pulse, temperature and blood pressure fluctuated, the limbs became soft and the gait was unstable, and even more serious, the hematopoietic system was damaged, causing death.

National standard for detection of vomitti

The national standard for the detection of vomittin is divided into feed and food, among which the limit standard for vomittin in feed is: 

Pig formula feed ≤ 1000 μ g/kg

Formula feed for calves ≤ 1000 μ g/kg

Animal formula feed in lactation period ≤ 1000 μ g/kg

Cattle formula feed ≤ 5000 μ g/kg

Poultry formula feed ≤ 5000 μ g/kg 

Limit standard for food vomittin

orn, corn flour, (residue, chips) ≤ 1000 μ g/kg

Barley, wheat, cereal, wheat flour 1000 μ g/kg 

Detection principle of vomiting toxin ELISA kit

The vomittin ELISA test kit adopts competitive ELISA, pre encapsulates vomittin antigen on the microplate, and adds sample/vomittin standard solution and vomittin antibody. The vomittin in the sample or standard solution competes with the vomittin antigen pre coated on the plate hole to bind the vomittin antibody. The unconjugated antibody is removed during washing. The enzyme labeled secondary antibody can react with the antibody, and then develop the color through the enzyme specific chromogenic agent, because the enzyme can change the colorless chromogenic agent into blue, the addition of the termination solution can stop the color development, and the blue can change into yellow. The absorption value can be measured at 450nm. The absorbance value of the sample was negatively correlated with the content of vomittin antigen in the residue. The content of vomittin in the corresponding residue can be obtained by comparing with the standard curve.

This product is a mycotoxin ELISA kit for reference only. Other mycotoxin related kits are also produced. For details, please call.

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